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Archaea are ubiquitous single-cell microorganisms that have often adapted to harsh conditions and play important roles in biogeochemical cycles with potential applications in biotechnology.

Methanococcus maripaludis, a methane-producing archaeon, is motile through multiple archaella on its cell surface. The major structural proteins archaellins of the archaellum are glycoproteins, modified with N-linked tetrasaccharides that are essential for the proper assembly and function of archaella.

The aglW gene, encoding the putative 4-epimerase AglW, plays a key role in the synthesis of the tetrasaccharide. The goal of our work was to biochemically demonstrate the 4-epimerase activity of AglW, and to develop assays to determine its substrate specificity and properties.

The glycosyltransferase-coupled assays are generally useful for the identification and specificity studies of novel 4-epimerases. Abstract Archaea are ubiquitous single-cell microorganisms that have often adapted to harsh conditions and play important roles in biogeochemical cycles with potential applications in biotechnology.

Publication types Research Support, Non-U.In this study, we used biochemistry and cell biology analyses to identify multi-protein complexes associated with A-motility. Here we characterized AgmU, a protein that localized to both the periplasm and cytoplasm. On firm surfaces, AgmU-mCherry co-localized with AglZ as distributed clusters that remained fixed with respect to the substratum as cells moved forward. Cluster formation was favored by hard surfaces where A-motility is favored. In contrast, AgmU-mCherry clusters were not observed on soft agar surfaces or when cells were in large groups, conditions that favor S-motility.

These proteins, important for the correct localization of AgmU and AglZ, appear to be organized as a motility complex, spanning the cytoplasm, inner membrane and the periplasm. Identification of this complex may be important for uncovering the mechanism of A-motility. Myxococcus xanthus is a rod-shaped Gram-negative soil bacterium with a complex life cycle that includes predation, vegetative growth, and development fruiting body formation.

During vegetative growth, M.

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When nutrients or prey are scarce, M. Directed motility is essential for vegetative swarming, predation, and development. Hodgkin and Kaiser, over thirty years ago, showed that M. One system, called social S -motility, is required for the movement of cells in groups and is now known to be powered by the retraction of polar Type IV pili, similar to twitching motility in Pseudomonas aeruginosa Li et al.

The other system, called adventurous A -motility, is required for the movement of isolated cells Zusman et al. However, the mechanism of A-motility is still unknown, although several hypotheses have been proposed Mignot et al. These two motility systems enable M. With both motility systems, cells periodically reverse their gliding direction. During reversals, the polarity of the cells is inverted so that the leading cell pole becomes the lagging cell pole and the old lagging cell pole becomes the new leading cell pole.

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The coordination of the two motility systems is essential for directed motility. The reversal frequencies of both the S- and A-motilities are regulated by the frizzy Frz chemosensory pathway Zusman et al. Little is known about how these two motility systems are coordinated or how cells switch between these systems as they encounter different surfaces. Mignot et al. Although these two models are very different, both require a protein complex spanning the cell envelope to power A-motility.He is aglowtoo, remembering Freud presiding over the Vienna Society.

Other coats were quiet and subdued from the back and aglow with Spirograph patterns on the front. She wears no make-up, her eyes are glassy, and her feverish cheeks are aglow. The great avenue of Hurston was all aglow with the golden sunset. A smile was on her happy face, her cheeks were aglow and her eyes were dancing in childish delight. Leilas face was aglow with a new-found happiness as she and Marjorie ran up the stairs to Veras room.

She was still young, and her skin, always beautiful, was aglow with the heat of the bath and the friction of the scrubbing. The speaker rose from her seat and stood erect, her face all aglow with noble emotion.

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If so, see if you can dig up the meanings of these vocab words from the popular novel. Words nearby aglow aglintaglistenaglitteraglossiaaglossostomiaaglowagluagluconaglutitionaglyconaglycosuria. Words related to aglow sunnybrightshinyluminousrichgoldenglisteningvividbrilliantshimmeringradiantdazzlingblazingsparklingintenseflashingsilveryaflamefloridvibrant. Example sentences from the Web for aglow He is aglowtoo, remembering Freud presiding over the Vienna Society.

The Catholic World, Vol. X, October Various. The Spoilers of the Valley Robert Watson. The Soul of a Child Edwin Bjorkman. Manasseh Maurus Jokai.The Series heavy-duty exit device provides superior durability while the smooth reduced projection touchbar assembly minimizes catch hazards.

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Product downloads Architectural documents 4. Barrier Free Exit Device Brochure 1. Antimicrobial Protected Door Hardware Brochure 1. Catalogs 1. Certifications 6. ES DE 2. DE 5. Parts and diagrams 7. Technical Brochure 1.All Florida Department of Agriculture and Consumer Services offices are currently closed to the public until further notice.

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Need more information? Live Chat Contact Us.You may take early prices or show prices in straight forecasts when available, in a fixed price forecast. Where a client selects combination forecasts A B C and stakes for 6 bets this will be settled as 6 straight forecasts as follows:Should any forecast contain a non-runner then the total stake will be placed to Win on the other selection.

In fixed price forecasts the remaining selection will be settled at SP. In races where a horse finishes alone and no forecast dividend is returned then all forecast bets nominating that horse to finish first will be settled as a Win single at SP on the winning horse.

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All other forecast bets in the race are lost. In the event of two or more horses dead-heating for first or second place then separate dividends will be declared and paid to each qualifying forecast.

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In fixed price forecasts the full odds will be paid with the stake split according to the number of horses which dead-heat. Tricast BettingYou may take early prices or show prices in straight Tricasts when available, in a fixed price Tricast. This is available on all horse races of 8 or more runners.

However, if no computer Tricast dividend is declared (e. The following applies to both Tricasts and fixed price Tricasts: if one selection is a non-runner then the bet will be settled as a straight forecast at the computer forecast dividend.

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If there are two non-runners then the bet will be settled as an SP single on the remaining selection. In the event of two or more horses dead-heating for first, second or third place then separate dividends will be declared and paid to each qualifying tricast.

In fixed price tricasts the full odds will be paid with the stake split according to the number of horses which dead-heat. Tricasts are accepted for singles only. Rule 4 (Deductions) will apply. ReservesIn races with reserves, any bets taken prior to a reserve horse being declared to run and if the reserve was not priced up at the time the bet was placed, then any such bets will be settled on the result 'without the reserve runner(s)'. Each way bets settled on the result "without the reserve runner(s)" will be based on the number of runners, excluding reserves, that start the race.

In races where reserves are priced but do not run, no Rule 4 (Deductions) will be applicable. Withdrawals Other Than AntePostWhere a horse is withdrawn before coming under starter's orders, or is officially deemed by the starter to have taken no part in the race, then stakes will be returned on the withdrawn horse and winning bets will be subject to deductions in accordance with Rule 4 (Deductions).

Should a horse be withdrawn and a new market formed then any bets laid at show prices prior to the new show will be subject to the above deductions. In the event of a further withdrawal after the market has been reformed then bets placed at show prices in the original market will be subject to a further deduction based on the price of the withdrawn horse in the original market.

Bets placed in the new market will be subject to a deduction based on the current price.

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The above scale will also apply in the case of non-runners in early price races and will be used for other events where we specifically advertise that deductions in line with Rule 4 (Deductions) will apply. ReRunsIn the event of a false start, etc. Returns on the remaining runners subject to Rule 4 (Deductions). The number of runners taking part in the rerun governs Place terms.

Walkovers and Void Horse RacesWalkovers and void races count as races but any horse so involved will be treated as a non-runner for settlement purposes. Normal place terms will apply for Each-way betting. In the event of a game being decided by a penalty shootout, then one goal will be added to the winning team's score and the game total for settlement purposes.

In 2-Way markets Push rules apply.

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Highest Scoring Period - If 2 or more periods have the same score, Tie will be settled as the winner. For settlement purposes the 51-60 minute duration will not include overtime if played. For settlement purposes the following website and box score details will be used to determine settlement times:Denmark www. Settlement of match-up bets will be determined by positions in the Official Final Ranking at the conclusion of the specified Tournament.

If teams are still ranked level, bets will be void. All-in, play or not. To Qualify From Group - Team to progress to Qualifying Round from Preliminary Round will be deemed winner. NHL To Win Conference - The team that progresses to the Stanley Cup Final will be deemed the winner.

Series winner is team with highest combined score from both tests.You can also use curl to customize a new logistic regression. Once a logistic regression has been successfully created it will have the following properties.

The coefficients output field is an array of pairs, one pair per class. The first element in the pair is a class value, and the second element is a nested array of coefficients for the logistic model that gives the probability of that class. Each inner array within the nested array contains the group of coefficients that pertain to a single input field. The class-coefficient pairs are listed in the same order as the class values in the objective field summary.

If the model was trained with missing values in the objective field, then a vector of coefficients will also be created for the missing class value, labeled with "", and listed last. These are found under a field named stats. It is possible for null to appear among the values contained in stats. Wald test statistics cannot be computed for zero-value coefficients, and so their corresponding entries are null.

Moreover, if the coefficients' information matrix is ill-conditioned, e. Creating a logistic regression is a process that can take just a few seconds or a few days depending on the size of the dataset used as input and on the workload of BigML's systems.

The logistic regression goes through a number of states until its fully completed. Through the status field in the logistic regression you can determine when the logistic regression has been fully processed and ready to be used to create predictions. Thus when retrieving a logisticregression, it's possible to specify that only a subset of fields be retrieved, by using any combination of the following parameters in the query string (unrecognized parameters are ignored): Fields Filter Parameters Parameter TypeDescription fields optional Comma-separated list A comma-separated list of field IDs to retrieve.

To update a logistic regression, you need to PUT an object containing the fields that you want to update to the logistic regression' s base URL. Once you delete a logistic regression, it is permanently deleted.

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If you try to delete a logistic regression a second time, or a logistic regression that does not exist, you will receive a "404 not found" response. However, if you try to delete a logistic regression that is being used at the moment, then BigML. To list all the logistic regressions, you can use the logisticregression base URL. By default, only the 20 most recent logistic regressions will be returned. You can get your list of logistic regressions directly in your browser using your own username and API key with the following links.

You can also paginate, filter, and order your logistic regressions. If every weight does end up zero (this is possible with sampled datasets), then the logistic regression creation will fail.

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Clusters can handle numeric, categorical, text and items fields as inputs: Numeric fields: the Eucledian distance is computed between the instances numeric values. Categorical fields: a common way to handle categorical data is to take each category as a new field and assign 0 or 1 depending on the category.

So a field with 20 categories will become 20 separate binary fields. Text and item fields: each instance is assigned a vector of terms and then cosine similarity is computed to determine closeness between instances.

Each cluster group is represented by a centroid or center that is computed using the mean for each numeric field and the mode for each categorical field. For text and items fields each centroid contains the terms or items which minimize the average cosine distance between the centroid and the points in its neighborhood. To create a cluster, you can select an arbitrary number of clusters (i.

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